Quikchange primer design

NEBaseChanger

NEBaseChanger can design primers specific to the mutagenesis experiment you are performing and calculate a recommended custom annealing temperature.

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(2) Quickchange provides less significant amplification of the target DNA sequence than conventional PCR. Therefore, extra care should to be ...

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Calculate a primer's melting temperature for the ...

The equation used for the melting temperature is: Tm = 81.5 + 0.41(%GC) - 675/N - % mismatch, where N = total number of bases. Paste your primer sequence below ...

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The QuikChange® Primer Design Program supports mutagenic primer design for your QuikChange mutagenesis experiments. Using primer design guidelines described ...

QuikChange Site Directed Mutagenesis. Primer Design Guidelines o Both of the mutagenic primers must contain the desired mutation and anneal to the same ...

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To create one or more base-pair mutations in the product DNA, primers that have a slight mismatch to the original template can be used. At a low ...

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